uv sterilizers (TSA? ) and UVB-radiation (4 J/cm2) were analyzed as sterilization methods. Sterilization with some sort of dose of a few. 75% hydrogen peroxide triggered a complete inactivation of vegetative cells and spores in most circumstances. All tests with UV-radiation were considerably more or less prosperous. A dose involving 100% did not show any considerable reduction in inactivation potential (inactivation efficiencies for vegetative cells one. 00-0. 98). These kinds of results indicate that hydrogen peroxide is definitely superior as a new germicidal agent inside UV-sterilizers. As opposed, UV-sterilizers are better appropriate for sterilizing bacteria spores. It had been found that UV-radiation is capable of inactivating bacterial spores. UV-radiation which has a dose regarding 100% caused the significant reduction in the inactivation efficiency regarding spores (0. 65-0. 10). In conclusion, UV-sterilization might become a great useful alternative with regard to the sterilization involving liquids or areas, where the make use of of a chemical substance germicidal agent is not permissible. For the similar reason, UV-sterilizers might be employed in the particular health care industry or in a spa in order to disinfect medical or even cosmetic tools plus instruments. Yet , the high UV-radiation dose must be regarded carefully as it might damage components and instruments. To our knowledge, this is the first report on the inactivation associated with *B. longum* subsp. *longum* with UV-radiation.

Numerous studies have shown that typically the inactivation of vegetative cells is just unfinished and the spores remain viable and are also able to colonize new surfaces ([@b3-bmj-44-4-1181]). Throughout this context, the particular present work is a first approach to investigate the inactivation of *B. longum* subsp. *longum* along with UV-radiation and the comparison of diverse disinfection systems.

Sanitation with hydrogen peroxide was found to get more effective intended for the complete eradication of vegetative cellular material and spores. As a result, hydrogen peroxide ought to be used often as an effective disinfection method. Regarding UV-radiation, the total inactivation of spores and the great inactivation of vegetative cells are feasible, too. However, typically the UV-sterilizers are fewer advantageous because their own UV-radiation capacity is usually limited along with the risk of the harming of materials is usually high. It is usually important to be aware that the UV-radiation regarding liquids (e. grams. milk or urine) in UV-sterilizers could be restricted in several situations because associated with absorption phenomena. UV-sterilizers equipped with the flow-through technique demonstrate same or better inactivation capacity involving liquids because involving a better utilization of the UV-radiation.

Generally, the investigated variables like time of exposure, UV-dose in addition to UV-radiation were certainly not found to get considerable for the inactivation of *B. longum* subsp. *longum*. However , a significantly increased inactivation efficiency has been observed with increased UV-radiation doses.

The particular present study was not possible without the support of typically the employees of the company "H�nsel & Heinz", Germany, specially Stephan Schultze. Moreover, we acknowledge typically the support by Mrs. Ingeborg Schultze-Heinzel (Wageningen, The Netherlands).

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